Phosphatase Inhibitor Cocktail II
Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to imporve the yield of native proteins is to add inhibitors of these aneymes known to be present in the source material. This phosphatase inhibitor cocktail has been optimized and tested for tyrosine protein phosphatases, acid and akaline phosphatase.
Application: On animal tissue and cell extracts with detergent.
Specificity: Tyrosine protein phosphatases, acid and alkaline phosphatases.
1. Reconstitute Phosphatase Inhibitor Cocktail II with 1 mL of water. Dilution at 1:100 is recommended.
2. Mix the diluted liquid with proper lysate.
3. Proceed with detection according to your research
2. Mix the diluted liquid with proper lysate.
3. Proceed with detection according to your research